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Fırat Ü.Tıp Fak.Göz Hast.A.D., Elazığ Purpose: The purpose of this study is evaluating the protective effect of melatonin on retinal ischemia-reperfusion (I/R) injury in guinea pigs.
Methods: Thirty male guinea pigs weighing 400-600 gr were divided into 3 groups with randomly selected. Group 1 (control group) was not applied any procedure, only to determine normal values, group 2 (I/R group) was applied ischemia-reperfusion and received serum physiologic as placebo, group 3 (melatonin group) was applied ischemi-reperfusion and received melatonin. Retinal ischemia was induced to group 2 and 3 by raising saline resorvoir to a height of 204 cm thereby increasing the intraocular pressure to 150 mmHg for 90 minutes. Ten minutes before the onset of ischemia, 1 ml serum physiologic injected subcutaneously to I/R group and 10 mg/kg melatonin injected subcutaneously to melatonin group. We repeated the same doses at 6^th, 12^th and 18^th hours. After 90 minutes of ischemia intraocular pressure reduced to normal values and reperfusion was performed for 24 hours, then eyes were enucleated.
Results: In the I/R group vacuolization and edema in photoreceptor layer, degenerative changes and edema of the ganglion cells were significantly higher than melatonin and. control group at histopathologic evaluation (p<0.01 and p<0.001) Between the control and melatonin groups there was not any significant difference about vacuolization and edema in photoreceptor layer, degenerative changes and edema of the ganglion cells (p>0.05).
Conclusion: According to histopathologic evaluation in retinal I/R induced eyes, melatonin decreased and limited the cellular damage. Keywords : Melatonin, Ischemia-reperfusion, Retina