2Assistant Prof. MD., Baskent University Histology and Embryology Department, Ankara, Turkey
3Prof. MD., Baskent University, Pathology Department, Ankara, Turkey
4Prof. MD., Baskent University, Histology and Embryology Department, Ankara, Turkey
5Ophthalmologist, Baskent University, Veterinary Faculty, Ankara, Turkey
6Prof. MD., Baskent University, Ophthalmology Department,Ankara, Turkey DOI : 10.37845/ret.vit.2020.29.33 Purpose: To evaluate the effect of intravitreal (IV) Matrine (Mat) injection on retinal endothelial cell proliferation (REP), retinal morphology, and apoptotic activity in hyperoxia induced (HIR) in-Vivo ROP- mouse model using C57BL/6J.
Methods: Total of 48 C57BL/6J mice were divided in 6 group each including 8. Group A and B-S exposed to room air were negative control groups; group C, D-S, E and F exposed to 75% ± 2% oxygen from postnatal day (PD) 7 to PD 12 comprised HIR groups. Group C and D-S were control groups, group E and F were low and high dose IV-Mat injected groups. Group A and C received no IV injection, B-S and D-S received 1 ?L IV sterile 0.9% NaCl injection, E and F received 0.78?g and 6.25?g IV-Mat injection respectively. After enucleation, we measured REP by counting neovascular tufts in cross-sections and examined histological, ultrastructural changes via light and transmission electron microscope. Apoptosis was detected using terminal deoxynucleotidyl transferase-mediated nick end-labeling.
Results: No REP was detected in negative control groups. REP levels were signifi cantly different between low- and high-dose Mat groups. REP levels were significantly lower in the Mat groups, compared to the control groups. The incidence of mitochondrial dysmorphology and apoptosis were lower in groups receiving Mat compared to the control groups.
Conclusion: Mat seems to suppress REP and has anti-apoptotic activity in the HIR mouse model.
Keywords : Retinopathy of prematurity, in-vivo-ROP-Mouse-Model, Matrine, Neovascularization, Apoptosis